HashClone is a new algorithm developed to identify the set of clonality markers during the patient follow-up in order to quantify the minimal residual disease.
Recently, the evaluation of the minimal residual disease (MRD) acquired a huge emphasis considering its role in the evaluation of the lymphoid malignancies. The assessment of MDR has shown to be used for treatment intervention in childhood and adult acute lymphoblastic leukemias (ALLs). The survival rate of ALL patients can be improved thanks to an accurate diagnosis and a careful therapeutic stratification according to the rearrangement of immunoglobulin heavy chain (IGH) and T-cell receptor (TCR) genes.
Many of the chromosome rearrangements in B-cell and T-cell tumors involve sequences implicated in complex and dynamical recombinations of the variable (V), diversity (D) and joining (J) segments of the immunoglobulin and T-cell receptor genes. The introduction of Next Generation Sequencing technology to analyze the junctional regions gain to reach an high level of sensitivity.
HashClone is a new algorithm developed to analyze each sample' patient from at diagnosis to each follow-up available. Hash Clone is a C++ open-source program. The output generated from HashClone (i.e. .gui file) can be visualized in the visualization page.